RIKEN Center for Advanced Photonics, Live Cell Super-Resolution Imaging Research Team

We elucidate molecular mechanisms of protein sorting and transport in membrane traffic

Topics

List of topics

August 31, 2024: The collaborative paper with Yoshinari et al.（Plant Cell Physiol.）"Rapid vacuolar sorting of the borate transporter BOR1 requires the adaptor complex AP-4 in Arabidopsis" has been published.

August 29, 2024: The collaborative paper with Suda et al.（iScience）"Remodeling of the secretory pathway is coordinated with de novo membrane formation in budding yeast gametogenesis" has been published.

July 11, 2024: The collaborative paper with Yagi et al.（Cell Struct. Funct.）"Deciphering the sub-Golgi localization of glycosyltransferases via 3D super-resolution imaging" has been published.

June 24, 2024: The paper by Miyashiro et al.（Frontiers Cell Dev. Biol.）"Extremely high spatiotemporal resolution microscopy for live cell imaging by single photon counting, noise elimination, and a novel restoration algorithm based on probability calculation” has been published.

May 27, 2024: The collaborative paper with Harada et al.（Nat. Comm.）"Dynamic movement of the Golgi unit and its glycosylation enzyme zones” has been published.

March 19, 2024: The paper by Tojima et al.（eLife）"Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast” has been published.

January 20, 2024: An article on the Golgi project by Nakano Team has been published in Impact Journal.

August 23, 2023: The collaborative paper with Obata et al.（Cell Rep.）"Golgi retention and oncogenic KIT signaling via PLCg2-PKD2-PI4KIIIb activation in gastrointestinal stromal tumor cells" has been published.

July 21, 2023: The collaborative paper with Toshima et al.（eLife）"The yeast endocytic early/sorting compartment exists as an independent sub-compartment within the trans-Golgi network" has been published.

April 1, 2023: Due to the age rule, Nakano steps down from the Team Leader position and Center Director Midorikawa serves as TL concurrently. Nakano stays in the team as a Deputy Team Leader.

April 7, 2021: The paper by Shimizu et al. (Nat. Commun.) was selected as an Editors' Highlight.

August 2, 2019: The paper by Tojima et al. (J. Cell Sci.) was selected for First Person Interview.

July 9, 2019: The paper by Kurokawa et al. (J. Cell Biol.) was selected as an outstanding article by JCB and appeared in July Highlights from JCB: Lipid and Membrane Biology Collection.

May 17, 2019: The paper by Kurokawa et al. (J. Cell Biol.) was described as Research Highlight in RIKEN Research.

Feb 1, 2019: Webpage of the NAKANO Laboratory was renewed.

Research

We are studying membrane trafficking, the molecular mechanisms of protein transport and sorting and the roles of membrane traffic in higher-order functions of multicellular organisms, plants in particular.

Subjects

Subject1

Membrane Traffic in Yeast Cells

Subject2

Membrane Traffic in Plant Cells

Subject3

Membrane Traffic in Animal Cells

Subject4

Development of High-speed and High-resolution Live Imaging Microscopy That Is Needed for Membrane Traffic Research

Projects

: JSPS Grant-in-Aid for Scientific Research (S) FY2018-FY2022
Full elucidation of sorting mechanisms in and around the Golgi apparatus by super-resolution live imaging
PI: Akihiko Nakano (RIKEN Center for Advanced Photonics)

: MEXT Grant-in-Aid for Scientific Research on Innovative Areas FY2017-FY2021
Toward an integrative understanding of functional zones in organelles
Leader: Shigeomi Shimizu (Tokyo Medical and Dental University)http://organellezone.org/english/
Study of the sorting zone for GPI-anchored proteins at ER exit sites
PI: Akihiko Nakano (RIKEN Center for Advanced Photonics)

Past projects

Latest Publications

*Akihiko Nakano (2022). The Golgi apparatus and its next-door neighbors. Frontiers Cell Dev. Biol. 10:884360.

Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Atsuko Ikeda, Misako Araki, Kouta Okazaki, Miyako Nakano, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Miho Waga, Akihiko Nakano, Kazuo Kurokawa, Manuel Muñiz, and *Koichi Funato (2022). Quality-controlled lipid-based protein sorting into selective ER exit sites. Cell Rep. 39:110768.

Yutaro Shimizu, Junpei Takagi, Emi Ito, Yoko Ito, Kazuo Ebine, Yamato Komatsu, Yumi Goto, Mayuko Sato, Kiminori Toyooka, Takashi Ueda, Kazuo Kurokawa, *Tomohiro Uemura, and *Akihiko Nakano (2021). Cargo sorting zones on the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants. Nat. Commun. 12:1901.