The SCLIM (super-resolution confocal live imaging microscopy) system we developed enables true live imaging of membrane trafficking, which involves very high-speed movement of very small vesicles. Our SCLIM2M model comprises high-speed spinning-disk confocal scanner (2000 frames/sec), high-speed Z-axis drive controller (no intervals), 3-color simultaneous spectroscopic unit (green, red and infrared), cooled high-S/N image intensifiers (10⁶-fold amplification), and high-speed and high-precision CMOS cameras (4 million pixels, 1000 frames/sec). By photon counting and Gaussian fitting to remove most of noises and by a novel deconvolution algorithm based on probability calculation we developed, it achieves resolutions at 20 volumes/sec in time and at < 80 nm in space.